Hotspot

trnS-GGA-rps4

LSC · 45153-45462 · 310 bp

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Pi 0.0034

MarkerSeek score 32.90

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 310
variable_sites: 3
pi: 0.0034
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	GGGTTCGAATCCCTCTCTCT	AGTAAGTGGGTCGGTTTGAA	55.0	45.0	58.2	56.7	399-401 bp	1.0	68.6
trnS-GGA-rps4_p2	ATGTAGGAGAGATGGCCGAG	AGTAAGTGGGTCGGTTTGAA	55.0	45.0	58.4	56.7	468-470 bp	1.0	67.2
trnS-GGA-rps4_p3	TAGGCTTTTGCTTACCGAGG	AGTAAGTGGGTCGGTTTGAA	50.0	45.0	57.6	56.7	417-419 bp	1.0	67.1
trnS-GGA-rps4_p4	GGGTTCGAATCCCTCTCTCT	AGTAAGTGGGTCGGTTTGAAA	55.0	42.9	58.2	57.4	399-401 bp	1.0	66.6
trnS-GGA-rps4_p5	TTTTGCTTACCGAGGGTTCG	AGTAAGTGGGTCGGTTTGAA	50.0	45.0	58.5	56.7	412-414 bp	1.0	66.4