Hotspot

ycf3-trnS-GGA

LSC · 44211-45065 · 855 bp

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Pi 0.000595
MarkerSeek score 44.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
855
variable_sites
2
pi
0.000595
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 989-1010 bp 1.0 66.0
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 990-1011 bp 1.0 66.0
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1027-1048 bp 1.0 65.8
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1028-1049 bp 1.0 65.8
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA AGTTCCAATGCTACGCCTTC 45.0 50.0 56.4 58.3 991-1012 bp 1.0 65.5