Hotspot

psaA-ycf3

LSC · 41561-42266 · 706 bp

Back to Dendrosenecio

Pi 0.000259

MarkerSeek score 48.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 1
pi: 0.000259
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AATGACCTGGTCTAGCCCAT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	889-894 bp	1.0	77.7
psaA-ycf3_p2	ATGACCTGGTCTAGCCCATT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	888-893 bp	1.0	77.7
psaA-ycf3_p3	AATGACCTGGTCTAGCCCAT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.1	58.3	891-896 bp	1.0	77.7
psaA-ycf3_p4	ATGACCTGGTCTAGCCCATT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.1	58.3	890-895 bp	1.0	77.7
psaA-ycf3_p5	ATGACCTGGTCTAGCCCATT	AAGCCGCTGAGTATTGGAAA	50.0	45.0	58.1	57.5	889-894 bp	1.0	75.9