Hotspot

psbK-psbI

LSC · 7941-8371 · 431 bp

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Pi 0.00321

MarkerSeek score 36.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 4
pi: 0.00321
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCTGGATTACG	50.0	50.0	57.9	57.1	566-568 bp	1.0	72.9
psbK-psbI_p2	TATGCCAGTCATACCCCTGT	TCTTCACGTCCTGGATTACG	50.0	50.0	57.8	57.1	606-608 bp	1.0	71.8
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCTGGATTACG	45.0	50.0	58.3	57.1	563-565 bp	1.0	70.9
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCTGGATTACG	45.0	50.0	58.3	57.1	564-566 bp	1.0	70.9
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCTGGATTACG	45.0	50.0	58.3	57.1	565-567 bp	1.0	70.9