Hotspot

rps16-trnQ-UUG

LSC · 6375-7332 · 958 bp

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Pi 0.00175
MarkerSeek score 44.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
958
variable_sites
5
pi
0.00175
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1031-1032 bp 1.0 78.7
rps16-trnQ-UUG_p2 AGAATAAGAATCCACGCCGG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 1063-1064 bp 1.0 77.8
rps16-trnQ-UUG_p3 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1046-1047 bp 1.0 76.8
rps16-trnQ-UUG_p4 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1045-1046 bp 1.0 76.8
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1035-1036 bp 1.0 73.9