Hotspot

rps2

LSC · 16949-17659 · 711 bp

Back to Dendropanax

Pi 0.00281

MarkerSeek score 60.50

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 3
pi: 0.00281
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ACCAATCACATAATATCCCCAGT	GCTTGGGTCGTGTATCTACG	39.1	55.0	57.1	58.2	948-949 bp	1.0	47.6
rps2_p2	ACCAATCACATAATATCCCCAGT	GCGGTAGAAGAGAAGGTTCC	39.1	55.0	57.1	57.7	920-921 bp	1.0	46.5
rps2_p3	ACCAATCACATAATATCCCCAGT	ACGGCTAATTTGCGGTAGAA	39.1	45.0	57.1	57.6	931-932 bp	1.0	45.8
rps2_p4	ACCAATCACATAATATCCCCAGT	GGGTCGTGTATCTACGGCTA	39.1	55.0	57.1	58.4	944-945 bp	1.0	45.7
rps2_p5	ACCAATCACATAATATCCCCAGT	CTTGGGTCGTGTATCTACGG	39.1	55.0	57.1	57.5	947-948 bp	1.0	45.0