Hotspot

rps16

LSC · 5267-6385 · 1119 bp

Back to Dendropanax

Pi 0.000596

MarkerSeek score 32.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1119
variable_sites: 1
pi: 0.000596
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TAGGATCAACCAACTGGGGT	AGGTGGGGTCTTGCTAAGAT	50.0	50.0	57.9	58.0	1483-1538 bp	1.0	82.7
rps16_p2	TAGGATCAACCAACTGGGGT	AAGTCGCAGATGGGTCAATC	50.0	50.0	57.9	58.0	1638-1694 bp	1.0	82.4
rps16_p3	CAAAGCGAATCCAACGAACC	AGGTGGGGTCTTGCTAAGAT	50.0	50.0	58.0	58.0	1888-1941 bp	1.0	82.1
rps16_p4	CAAAGCGAATCCAACGAACC	AAGTCGCAGATGGGTCAATC	50.0	50.0	58.0	58.0	2043-2097 bp	1.0	82.0
rps16_p5	CAAAGCGAATCCAACGAACC	GAAGTCGCAGATGGGTCAAT	50.0	50.0	58.0	58.0	2044-2098 bp	1.0	82.0