Hotspot

rpl33

LSC · 70112-70312 · 201 bp

Back to Dendropanax

Pi 0.00663

MarkerSeek score 42.10

Primer available yes

Label rpl33

Feature Metadata

label_name: rpl33
feature_type: gene
region: LSC
length_bp: 201
variable_sites: 2
pi: 0.00663
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33_p1	TTTTCCCAGATGCGTTGACA	GAGTTCCCCGGAGAATGAAC	45.0	55.0	58.0	58.0	1398-1406 bp	1.0	80.5
rpl33_p2	TTTTCCCAGATGCGTTGACA	GTTCCCCGGAGAATGAACTC	45.0	55.0	58.0	58.0	1396-1404 bp	1.0	80.5
rpl33_p3	TTTTCCCAGATGCGTTGACA	GGCTCTAAGACCAGCAGTTC	45.0	55.0	58.0	58.0	1141-1144 bp	1.0	80.1
rpl33_p4	ATGCGTTGACATTCCCCTTT	GGCTCTAAGACCAGCAGTTC	45.0	55.0	58.1	58.0	1132-1135 bp	1.0	79.7
rpl33_p5	TTTTCCCAGATGCGTTGACA	GGGTTACATACAGGTGCTCG	45.0	55.0	58.0	58.1	707-709 bp	1.0	79.6