Hotspot

rpl32-trnL(UAG)

SSC · 116219-117027 · 809 bp

Back to Dendropanax

Pi 0.00412

MarkerSeek score 61.40

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 809
variable_sites: 5
pi: 0.00412
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	936 bp	1.0	79.0
rpl32-trnL(UAG)_p2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	937 bp	1.0	79.0
rpl32-trnL(UAG)_p3	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	946 bp	1.0	78.1
rpl32-trnL(UAG)_p4	GCAGCCTTAAAAGCGTTGTC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	925 bp	1.0	77.9
rpl32-trnL(UAG)_p5	GCAGCCTTAAAAGCGTTGTC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	926 bp	1.0	77.9