Hotspot

petA-psbJ

LSC · 65427-66455 · 1029 bp

Back to Dendropanax

Pi 0.00259

MarkerSeek score 61.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 4
pi: 0.00259
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1168-1174 bp	1.0	77.5
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1151-1157 bp	1.0	76.5
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1165-1171 bp	1.0	76.3
petA-psbJ_p4	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1163-1169 bp	1.0	75.0
petA-psbJ_p5	TACGTGTCCAAGGCCTTTTG	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.4	57.6	1162-1168 bp	1.0	74.6