Hotspot

atpB-rbcL

LSC · 57036-57831 · 796 bp

Back to Dendropanax

Pi 0.00168

MarkerSeek score 59.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 2
pi: 0.00168
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGTGATATTCCCCAGGTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	919-922 bp	1.0	72.0
atpB-rbcL_p2	TGGGTGATATTCCCCAGGTT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	918-921 bp	1.0	71.7
atpB-rbcL_p3	CATCCAGTACCGGACCAATG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.0	58.0	942-945 bp	1.0	71.7
atpB-rbcL_p4	CATCCAGTACCGGACCAATG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.0	58.1	941-944 bp	1.0	71.4
atpB-rbcL_p5	GGAAACCCCAGAACCTGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	884-887 bp	1.0	71.3