Hotspot

psbM-trnD(GUC)

LSC · 31361-32519 · 1159 bp

Back to Dendropanax

Pi 0.00345

MarkerSeek score 61.20

Primer available yes

Label psbM-trnD(GUC)

Feature Metadata

label_name: psbM-trnD(GUC)
feature_type: igs
region: LSC
length_bp: 1159
variable_sites: 6
pi: 0.00345
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD(GUC)_p1	AGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.2	58.5	1289-1291 bp	1.0	62.5
psbM-trnD(GUC)_p2	AAGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.9	58.5	1290-1292 bp	1.0	60.0
psbM-trnD(GUC)_p3	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1268-1270 bp	1.0	58.9
psbM-trnD(GUC)_p4	AGCAGAAAAGCAGTAGGAACT	GTTCAATTGGTCAGAGCACC	42.9	50.0	57.2	57.0	1290-1292 bp	1.0	58.3
psbM-trnD(GUC)_p5	GCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.7	58.5	1288-1290 bp	1.0	58.1