Hotspot

trnK(UUU)-rps16

LSC · 4342-5266 · 925 bp

Back to Dendropanax

Pi 0.00383

MarkerSeek score 62.80

Primer available yes

Label trnK(UUU)-rps16

Feature Metadata

label_name: trnK(UUU)-rps16
feature_type: igs
region: LSC
length_bp: 925
variable_sites: 5
pi: 0.00383
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK(UUU)-rps16_p1	CGAATCCTTCGCTTCATCCA	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.1	58.3	1024-1079 bp	1.0	81.4
trnK(UUU)-rps16_p2	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	55.0	45.0	57.9	58.3	1037-1092 bp	1.0	81.2
trnK(UUU)-rps16_p3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	976-1031 bp	1.0	80.5
trnK(UUU)-rps16_p4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	975-1030 bp	1.0	80.5
trnK(UUU)-rps16_p5	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1061-1116 bp	1.0	80.0