Hotspot

ycf1

SSC · 122605-128217 · 5613 bp

Back to Dendrocnide

Pi 0.00526

MarkerSeek score 44.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5613
variable_sites: 54
pi: 0.00526
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAAAGTGACTCTGTTATAGTAT	ACTTTTTCGTTGAAAGGATACA	30.4	31.8	52.1	54.2	221-227 bp	1.0	45.0
ycf1_p2	TTAGAAAGTGACTCTGTTATAGT	ACTTTTTCGTTGAAAGGATACA	30.4	31.8	52.3	54.2	223-229 bp	1.0	44.9
ycf1_p3	TTAGAAAGTGACTCTGTTATAGT	AACTTTTTCGTTGAAAGGATACA	30.4	30.4	52.3	55.0	224-230 bp	1.0	44.9
ycf1_p4	ATTAGAAAGTGACTCTGTTATAGT	ACTTTTTCGTTGAAAGGATACA	29.2	31.8	53.0	54.2	224-230 bp	1.0	44.9
ycf1_p5	ACTAATATTAGAAAGTGACTCTGT	ACTTTTTCGTTGAAAGGATACA	29.2	31.8	53.0	54.2	230-236 bp	1.0	44.8