Hotspot

rps16

LSC · 4904-5882 · 979 bp

Back to Dendrocnide

Pi 0.01

MarkerSeek score 42.40

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 979
variable_sites: 17
pi: 0.01
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TTTTCGGCGCAATCAAAACA	CGTAGGTTCGAATCCTTCCG	40.0	55.0	57.8	58.2	1846-1877 bp	1.0	79.5
rps16_p2	GGTTGGTGATTAGGGTGGTG	CGTAGGTTCGAATCCTTCCG	55.0	55.0	58.2	58.2	1225 bp	0.5	57.4
rps16_p3	GTTGGTGATTAGGGTGGTGG	CGTAGGTTCGAATCCTTCCG	55.0	55.0	58.2	58.2	1224 bp	0.5	57.4
rps16_p4	GGTTGGTGATTAGGGTGGTG	GTTTTGGTCCCGGTATTCGT	55.0	50.0	58.2	58.2	1242 bp	0.5	57.2
rps16_p5	GTTGGTGATTAGGGTGGTGG	GTTTTGGTCCCGGTATTCGT	55.0	50.0	58.2	58.2	1241 bp	0.5	57.2