Hotspot

ndhI-ndhA

SSC · 118282-118363 · 82 bp

Back to Dendrocnide

Pi 0.0183

MarkerSeek score 55.90

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 82
variable_sites: 3
pi: 0.0183
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGCAGCTCGTACTGTTTGTT	GGCTAAGAATGGACCAGCTG	45.0	55.0	58.1	58.3	243-246 bp	1.0	78.0
ndhI-ndhA_p2	TTGCAGCTCGTACTGTTTGT	GGCTAAGAATGGACCAGCTG	45.0	55.0	58.1	58.3	244-247 bp	1.0	78.0
ndhI-ndhA_p3	TGCAGCTCGTACTGTTTGTT	GCTAAGAATGGACCAGCTGT	45.0	50.0	58.1	57.6	242-245 bp	1.0	77.3
ndhI-ndhA_p4	TTGCAGCTCGTACTGTTTGT	GCTAAGAATGGACCAGCTGT	45.0	50.0	58.1	57.6	243-246 bp	1.0	77.3
ndhI-ndhA_p5	TGCAGCTCGTACTGTTTGTT	AGGCTAAGAATGGACCAGCT	45.0	50.0	58.1	58.4	244-247 bp	1.0	77.3