Hotspot

ndhE-ndhG

SSC · 116623-116854 · 232 bp

Back to Dendrocnide

Pi 0.0216

MarkerSeek score 42.80

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 232
variable_sites: 9
pi: 0.0216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCCTCTGTTCTTGGAGCAGA	TCTTTGGACCGTTGGGAATG	50.0	50.0	58.0	58.1	1188 bp	1.0	80.5
ndhE-ndhG_p2	TTGCCTTTTAACTGTCGGCT	TGGGAATGGGGTTACTTTGC	45.0	50.0	58.0	58.1	658 bp	0.75	70.1
ndhE-ndhG_p3	TCCTCTGTTCTTGGAGCAGA	TGGGAATGGGGTTACTTTGC	50.0	50.0	58.0	58.1	1176 bp	0.75	69.9
ndhE-ndhG_p4	CTTAGCTTTACACCCGTCCC	TGGGAATGGGGTTACTTTGC	55.0	50.0	58.0	58.1	1147 bp	0.75	69.9
ndhE-ndhG_p5	GACAGGCGGATTCACATCTT	TGGGAATGGGGTTACTTTGC	50.0	50.0	58.0	58.1	1211 bp	0.75	69.8