Hotspot

petA-psbJ

LSC · 62317-63151 · 835 bp

Back to Dendrocnide

Pi 0.00838

MarkerSeek score 53.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 14
pi: 0.00838
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGCATACAAGGCCT	GCCGATACTACTGGAAGGAT	50.0	50.0	58.0	56.2	1072-1079 bp	1.0	66.4
petA-psbJ_p2	TCCATTACGCATACAAGGCC	GCCGATACTACTGGAAGGAT	50.0	50.0	58.0	56.2	1073-1080 bp	1.0	66.4
petA-psbJ_p3	GCCTTTTGCTCTTTTTGGCA	GCCGATACTACTGGAAGGAT	45.0	50.0	58.0	56.2	1056-1063 bp	1.0	66.3
petA-psbJ_p4	CCATTACGCATACAAGGCCT	GCCGATACTACTGGAAGGATT	50.0	47.6	58.0	57.0	1072-1079 bp	1.0	64.5
petA-psbJ_p5	TCCATTACGCATACAAGGCC	GCCGATACTACTGGAAGGATT	50.0	47.6	58.0	57.0	1073-1080 bp	1.0	64.5