Pi
0.0115
MarkerSeek score
51.40
Primer available
yes
Label
trnT-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 862-903 bp | 1.0 | 82.4 |
| trnT-trnL-UAA_p2 | GAGTCGAACCGATGACCATC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 893-934 bp | 1.0 | 82.1 |
| trnT-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 870-911 bp | 1.0 | 81.8 |
| trnT-trnL-UAA_p4 | GAGTCGAACCGATGACCATC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 901-942 bp | 1.0 | 81.5 |
| trnT-trnL-UAA_p5 | ATGGAGAAAAGCCCGCTATC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 914-955 bp | 1.0 | 81.2 |