Hotspot

rps4-trnT

LSC · 45630-46147 · 518 bp

Back to Dendrocnide

Pi 0.00579

MarkerSeek score 54.90

Primer available yes

Label rps4-trnT

Feature Metadata

label_name: rps4-trnT
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 6
pi: 0.00579
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT_p1	CGGGGCGTAATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	696-747 bp	1.0	81.0
rps4-trnT_p2	CGGGGCGTAATTGGTTTCTA	GATGGTCATCGGTTCGACTC	50.0	55.0	58.0	58.2	665-716 bp	1.0	80.9
rps4-trnT_p3	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	630-681 bp	1.0	80.6
rps4-trnT_p4	CCCCCAGACGACGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	57.9	58.2	599-650 bp	1.0	80.5
rps4-trnT_p5	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	608-659 bp	1.0	79.3