Hotspot

trnG-GCC-trnfM-CAU

LSC · 35638-35818 · 181 bp

Back to Dendrocnide

Pi 0.0239
MarkerSeek score 54.90
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
181
variable_sites
8
pi
0.0239
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 246-247 bp 1.0 82.1
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 247-248 bp 1.0 82.1
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 282-283 bp 1.0 81.9
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 283-284 bp 1.0 81.9
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC GCAGTTTGGTAGCTTGCAAG 50.0 50.0 58.1 57.9 275-276 bp 1.0 81.7