Hotspot

psbK-psbI

LSC · 6705-7148 · 444 bp

Back to Dendrocnide

Pi 0.0131
MarkerSeek score 44.60
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
444
variable_sites
11
pi
0.0131
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 TGGCAAGCTGCTGTAAGTTT AACAAATAGTTTTAGAGTAAGCA 45.0 26.1 58.3 52.1 494-530 bp 1.0 42.9
psbK-psbI_p2 TTGGCAAGCTGCTGTAAGTT AACAAATAGTTTTAGAGTAAGCA 45.0 26.1 58.3 52.1 495-531 bp 1.0 42.9
psbK-psbI_p3 CTTTTGTTTGGCAAGCTGCT AACAAATAGTTTTAGAGTAAGCA 45.0 26.1 58.1 52.1 502-538 bp 1.0 42.8
psbK-psbI_p4 GTTTGGCAAGCTGCTGTAAG AACAAATAGTTTTAGAGTAAGCA 50.0 26.1 57.9 52.1 497-533 bp 1.0 42.8
psbK-psbI_p5 TTTGGCAAGCTGCTGTAAGT AACAAATAGTTTTAGAGTAAGCA 45.0 26.1 58.3 52.1 496-532 bp 1.0 42.8