Hotspot

trnK-UUU-rps16

LSC · 4184-4903 · 720 bp

Back to Dendrocnide

Pi 0.00949

MarkerSeek score 50.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 720
variable_sites: 12
pi: 0.00949
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	GCTCAGCCTACAGGAACTTT	50.0	50.0	57.9	57.5	878-895 bp	1.0	78.3
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	GCTCAGCCTACAGGAACTTT	50.0	50.0	57.9	57.5	877-894 bp	1.0	78.3
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	GCTCAGCCTACAGGAACTTT	50.0	50.0	58.2	57.5	794-811 bp	1.0	77.7
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	GCTCAGCCTACAGGAACTTT	50.0	50.0	58.2	57.5	793-810 bp	1.0	77.7
trnK-UUU-rps16_p5	CAGTCGCGGTCTTACAAACT	GCTCAGCCTACAGGAACTTT	50.0	50.0	58.2	57.5	879-896 bp	1.0	77.0