Hotspot

trnH-GUG-psbA

LSC · 72-284 · 213 bp

Back to Dendrocnide

Pi 0.0125

MarkerSeek score 53.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 5
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TGGGCTGATATCATCAACCG	55.0	50.0	58.1	57.5	346-353 bp	1.0	76.6
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GGGCTGATATCATCAACCGT	55.0	50.0	58.1	57.4	345-352 bp	1.0	76.6
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TGGGCTGATATCATCAACCG	50.0	50.0	57.9	57.5	360-367 bp	1.0	76.5
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TGGGCTGATATCATCAACCG	50.0	50.0	57.9	57.5	359-366 bp	1.0	76.5
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TGGGCTGATATCATCAACCG	50.0	50.0	57.9	57.5	361-368 bp	1.0	76.5