Hotspot

rpl16

LSC · 80170-81678 · 1509 bp

Back to Dendrocalamus

Pi 0.0022

MarkerSeek score 35.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1509
variable_sites: 14
pi: 0.0022
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCAGCAATAGTGTCCTTGCC	GCCTTGTCTTCCCATTCTGT	50.0	50.0	57.9	57.8	2284-2307 bp	1.0	79.0
rpl16_p2	TAACCGCTGCATTGTCATCA	GCCTTGTCTTCCCATTCTGT	45.0	50.0	57.9	57.8	2013-2035 bp	1.0	78.7
rpl16_p3	TGATAACCGCTGCATTGTCA	GCCTTGTCTTCCCATTCTGT	45.0	50.0	57.9	57.8	2016-2038 bp	1.0	78.7
rpl16_p4	ACCGTCTTCGCATTTGAACT	GCCTTGTCTTCCCATTCTGT	45.0	50.0	58.1	57.8	1979-2001 bp	1.0	78.5
rpl16_p5	TTTCTAGAGGCATTTGGGGC	GCCTTGTCTTCCCATTCTGT	50.0	50.0	57.9	57.8	1917-1939 bp	1.0	78.4