Hotspot

rpl14

LSC · 79689-80060 · 372 bp

Back to Dendrocalamus

Pi 0.00179

MarkerSeek score 30.10

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 372
variable_sites: 5
pi: 0.00179
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	AGCATATACGTGAAACACAATCT	TCTTTCATCCTTTTGCATTGA	34.8	33.3	56.4	54.0	480 bp	1.0	41.0
rpl14_p2	AGCATATACGTGAAACACAATCT	TCTTTCATCCTTTTGCATTGAA	34.8	31.8	56.4	54.8	480 bp	1.0	41.0
rpl14_p3	AGCATATACGTGAAACACAATCT	TTCTTTCATCCTTTTGCATTGA	34.8	31.8	56.4	54.8	481 bp	1.0	41.0
rpl14_p4	AAGCATATACGTGAAACACAATCT	TCTTTCATCCTTTTGCATTGA	33.3	33.3	57.0	54.0	481 bp	1.0	41.0
rpl14_p5	AGCATATACGTGAAACACAA	TCTTTCATCCTTTTGCATTGA	35.0	33.3	53.0	54.0	480 bp	1.0	41.0