Hotspot

ndhD

SSC · 110338-111840 · 1503 bp

Back to Dendrocalamus

Pi 0.000768

MarkerSeek score 30.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 8
pi: 0.000768
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCAACTCTTTTGTGCGGATC	TTTAGGGCCTGAAACAACCC	50.0	50.0	58.0	58.0	2402-2405 bp	1.0	80.5
ndhD_p2	GCAACTCTTTTGTGCGGATC	ATGGGTGTAAAGCCAAGCAA	50.0	45.0	58.0	58.0	2511-2514 bp	1.0	80.4
ndhD_p3	GCAACTCTTTTGTGCGGATC	AAACCATTGCTTCGTCTGGT	50.0	45.0	58.0	58.0	2713-2716 bp	1.0	80.3
ndhD_p4	GCAACTCTTTTGTGCGGATC	TCTTGGCTCTTTTCACGCTT	50.0	45.0	58.0	58.0	2787-2790 bp	1.0	80.2
ndhD_p5	GCAACTCTTTTGTGCGGATC	TGGCTCTTTTCACGCTTTCT	50.0	45.0	58.0	58.0	2784-2787 bp	1.0	80.2