Hotspot

ycf4-cemA

LSC · 60506-60947 · 442 bp

Back to Dendrocalamus

Pi 0.00352

MarkerSeek score 48.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 17
pi: 0.00352
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	CCCAGGGCAAAAGTACGATA	45.0	50.0	58.1	57.3	534-547 bp	1.0	77.9
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	CCCAGGGCAAAAGTACGATA	45.0	50.0	57.8	57.3	550-563 bp	1.0	77.3
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	CCCAGGGCAAAAGTACGATAA	45.0	47.6	58.1	58.0	534-547 bp	1.0	75.4
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	CCCAGGGCAAAAGTACGATAA	45.0	47.6	57.8	58.0	550-563 bp	1.0	74.9
ycf4-cemA_p5	TGGCCTATTTCTTGCGTGTA	CCCAGGGCAAAAGTACGATA	45.0	50.0	57.2	57.3	533-546 bp	1.0	72.5