Hotspot

rbcL-psaI

LSC · 58232-59478 · 1247 bp

Back to Dendrocalamus

Pi 0.004

MarkerSeek score 54.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 25
pi: 0.004
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1384-1387 bp	1.0	79.0
rbcL-psaI_p2	CGCAGCTTGTGAAGTATGGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	1390-1393 bp	1.0	78.4
rbcL-psaI_p3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1363-1366 bp	1.0	78.4
rbcL-psaI_p4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.6	58.0	1368-1371 bp	1.0	78.1
rbcL-psaI_p5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.6	57.9	1369-1372 bp	1.0	77.8