Hotspot

trnY-GUA-trnD-GUC

LSC · 16813-17164 · 352 bp

Back to Dendrocalamus

Pi 0.00171

MarkerSeek score 38.90

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 6
pi: 0.00171
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	58.0	58.5	476-477 bp	1.0	76.8
trnY-GUA-trnD-GUC_p2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	443-444 bp	1.0	74.5
trnY-GUA-trnD-GUC_p3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	442-443 bp	1.0	74.3
trnY-GUA-trnD-GUC_p4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	50.0	50.0	58.0	57.0	475-476 bp	1.0	72.7
trnY-GUA-trnD-GUC_p5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.2	58.5	444-445 bp	1.0	71.1