Hotspot

psbZ-trnfM-CAU

LSC · 12219-13008 · 790 bp

Back to Dendrocalamus

Pi 0.00075
MarkerSeek score 48.90
Primer available yes
Label psbZ-trnfM-CAU

Feature Metadata

label_name
psbZ-trnfM-CAU
feature_type
igs
region
LSC
length_bp
790
variable_sites
5
pi
0.00075
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnfM-CAU_p1 TTGCTTCTCCTGATGGTTGG CGAGGCTCATAACCTTGAGG 50.0 55.0 57.8 57.8 919-948 bp 1.0 79.4
psbZ-trnfM-CAU_p2 GCTTCTCCTGATGGTTGGTC CGAGGCTCATAACCTTGAGG 55.0 55.0 58.3 57.8 917-946 bp 1.0 79.0
psbZ-trnfM-CAU_p3 TTGCTTCTCCTGATGGTTGG CAGTTTGGTAGCTCACGAGG 50.0 55.0 57.8 58.3 934-963 bp 1.0 78.9
psbZ-trnfM-CAU_p4 GCTTCTCCTGATGGTTGGTC CAGTTTGGTAGCTCACGAGG 55.0 55.0 58.3 58.3 932-961 bp 1.0 78.5
psbZ-trnfM-CAU_p5 TTGCTTCTCCTGATGGTTGG GAGCGGAGTAGAGCAGTTTG 50.0 55.0 57.8 58.4 947-976 bp 1.0 78.2