Hotspot

rpl16

LSC · 81131-82724 · 1594 bp

Back to Dendrobium

Pi 0.024

MarkerSeek score 72.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1594
variable_sites: 346
pi: 0.024
unique_haplotype_count: 69
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TATCATGCCGTTGTCACGTT	GCAGGGTTCCCTTACAAACA	45.0	50.0	57.9	58.0	2217-2346 bp	0.986111	90.7
rpl16_p2	TTGCCGGTTCCTATGATTCG	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.1	58.0	2075-2204 bp	0.972222	90.4
rpl16_p3	TGGGCACTGCTTCTTTGATT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2134-2263 bp	0.944444	89.7
rpl16_p4	TTGGGCACTGCTTCTTTGAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2135-2264 bp	0.944444	89.7
rpl16_p5	CGAGCAATCGCACCAAAAAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2300-2429 bp	0.902778	87.9