Hotspot

trnV-GAC-3'rps12

IRa · 133624-135580 · 1957 bp

Back to Dendrobium

Pi 0.0234
MarkerSeek score 83.70
Primer available yes
Label trnV-GAC-3'rps12

Feature Metadata

label_name
trnV-GAC-3'rps12
feature_type
igs
region
IRa
length_bp
1957
variable_sites
1016
pi
0.0234
unique_haplotype_count
65
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-3'rps12_p1 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1811-2143 bp 0.986111 88.1
trnV-GAC-3'rps12_p2 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1815-2147 bp 0.986111 88.0
trnV-GAC-3'rps12_p3 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1810-2142 bp 0.986111 88.0
trnV-GAC-3'rps12_p4 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1813-2145 bp 0.986111 87.1
trnV-GAC-3'rps12_p5 GTCAAGGTGACACTCTACCG TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1796-2128 bp 0.916667 83.8