Hotspot

psaC-rps15

SSC · 115520-118660 · 3141 bp

Back to Dendrobium

Pi 0.0324

MarkerSeek score 73.40

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 3141
variable_sites: 1214
pi: 0.0324
unique_haplotype_count: 68
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	AGACACCAGACGAATCAATGA	ACGAACTTATTCTATAACTAACCT	42.9	29.2	57.0	53.1	237-347 bp	0.847222	45.9
psaC-rps15_p2	TGTAGACACCAGACGAATCA	ACGAACTTATTCTATAACTAACCT	45.0	29.2	55.9	53.1	240-350 bp	0.833333	45.3
psaC-rps15_p3	TCTGTTTATGAGAAAGGGCCA	ACGAACTTATTCTATAACTAACCT	42.9	29.2	56.9	53.1	181-291 bp	0.833333	45.3
psaC-rps15_p4	TGAGAAAGGGCCAAGATACT	ACGAACTTATTCTATAACTAACCT	45.0	29.2	55.8	53.1	173-283 bp	0.833333	45.3
psaC-rps15_p5	TGTAGACACCAGACGAATCAA	ACGAACTTATTCTATAACTAACCT	42.9	29.2	56.7	53.1	240-350 bp	0.833333	45.3