Hotspot

rpl32-trnL-UAG

SSC · 111905-112634 · 730 bp

Back to Dendrobium

Pi 0.0413

MarkerSeek score 87.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 730
variable_sites: 260
pi: 0.0413
unique_haplotype_count: 68
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	656-870 bp	1.0	90.7
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	649-863 bp	1.0	90.2
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	655-869 bp	1.0	90.1
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	654-868 bp	1.0	90.1
rpl32-trnL-UAG_p5	AATCTGTTTCCACCGGACAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	664-878 bp	0.986111	89.0