Hotspot

psbB-psbT

LSC · 73426-73833 · 408 bp

Back to Dendrobium

Pi 0.0669

MarkerSeek score 77.90

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 218
pi: 0.0669
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCCAACTACAAGGAGACAAGT	ACCAATGCTTCCATAGATTCGA	42.9	40.9	56.2	57.6	257-685 bp	0.916667	48.7
psbB-psbT_p2	GATCCAACTACAAGGAGACAAGT	ACCAATGCTTCCATAGATTCGA	43.5	40.9	57.8	57.6	259-687 bp	0.916667	48.7
psbB-psbT_p3	ATCCAACTACAAGGAGACAAGT	ACCAATGCTTCCATAGATTCGA	40.9	40.9	56.7	57.6	258-686 bp	0.916667	48.7
psbB-psbT_p4	TCCAACTACAAGGAGACAAGT	ACCAATGCTTCCATAGATTCG	42.9	42.9	56.2	56.1	257-685 bp	0.916667	48.7
psbB-psbT_p5	TCCAACTACAAGGAGACAAGT	ACCAATGCTTCCATAGATTCGAT	42.9	39.1	56.2	58.0	257-685 bp	0.916667	48.7