Hotspot

atpB-rbcL

LSC · 52875-53893 · 1019 bp

Back to Dendrobium

Pi 0.0253

MarkerSeek score 78.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1019
variable_sites: 297
pi: 0.0253
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	974-1207 bp	1.0	90.0
atpB-rbcL_p2	ATCTTGCCCGGTGGAAAAAT	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.1	58.1	1006-1239 bp	0.986111	90.0
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	975-1208 bp	1.0	87.6
atpB-rbcL_p4	ATCTTGCCCGGTGGAAAAAT	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.1	58.4	1007-1240 bp	0.986111	87.6
atpB-rbcL_p5	ATCTTGCCCGGTGGAAAAAT	TCCAACGCTTGCTTTAGTCT	45.0	45.0	58.1	57.5	1034-1267 bp	0.972222	86.0