Hotspot

psbC-trnS-UGA

LSC · 35580-35712 · 133 bp

Back to Dendrobium

Pi 0.0452

MarkerSeek score 79.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 133
variable_sites: 47
pi: 0.0452
unique_haplotype_count: 47
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	238-617 bp	0.986111	90.0
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	218-597 bp	1.0	89.7
psbC-trnS-UGA_p3	AGCGGGGTTTGAAAAAGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.2	58.0	275-654 bp	0.986111	89.5
psbC-trnS-UGA_p4	AGCGGGGTTTGAAAAAGGAA	ATAGCCCCGGTCTTGAAAAC	45.0	50.0	58.2	57.9	255-634 bp	1.0	89.3
psbC-trnS-UGA_p5	TTCCATGACCCCTCTTAGCT	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.7	58.0	230-609 bp	0.986111	89.1