Hotspot

trnS-GCU-trnG-UCC

LSC · 8411-9452 · 1042 bp

Back to Dendrobium

Pi 0.0326
MarkerSeek score 81.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1042
variable_sites
372
pi
0.0326
unique_haplotype_count
67
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AACGGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 1018-1296 bp 1.0 78.9
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AACGGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 1017-1295 bp 1.0 78.9
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AAACGGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 1019-1297 bp 1.0 77.0
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC AAACGGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 1018-1296 bp 1.0 77.0
trnS-GCU-trnG-UCC_p5 CGGAGAGAGAGGGATTCGAA AACGGAAAGACCCCTTAACT 55.0 45.0 58.3 56.4 1071-1349 bp 1.0 76.4