Hotspot

ycf1

SSC · 124068-129377 · 5310 bp

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Pi 0.0138

MarkerSeek score 59.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5310
variable_sites: 439
pi: 0.0138
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGCTATTATCAACAAAGGCA	ACGATTATGGCGAGAAAGGG	38.1	50.0	55.5	57.8	192-207 bp	1.0	61.3
ycf1_p2	TGGCTATTATCAACAAAGGCA	AAGGGCGCGGAATTAAAGAT	38.1	45.0	55.5	57.9	214-223 bp	0.923077	59.4
ycf1_p3	TGGCTATTATCAACAAAGGCA	AGGGCGCGGAATTAAAGATT	38.1	45.0	55.5	57.9	213-222 bp	0.923077	59.4
ycf1_p4	TGGCTATTATCAACAAAGGCAA	AAGGGCGCGGAATTAAAGAT	36.4	45.0	56.3	57.9	214-223 bp	0.923077	57.5
ycf1_p5	TGGCTATTATCAACAAAGGCAA	AGGGCGCGGAATTAAAGATT	36.4	45.0	56.3	57.9	213-222 bp	0.923077	57.5