Hotspot

rpl16

LSC · 81652-83180 · 1529 bp

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Pi 0.0136

MarkerSeek score 67.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1529
variable_sites: 131
pi: 0.0136
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTTTTCACAGGGCCCCTTTT	TGACTCCATATACCATATAATTGCT	45.0	32.0	58.1	55.5	1584-1607 bp	0.269231	11.9
rpl16_p2	TTTTTCACAGGGCCCCTTTT	TTGACTCCATATACCATATAATTGCT	45.0	30.8	58.1	56.1	1585-1608 bp	0.269231	11.9
rpl16_p3	TTTTTCACAGGGCCCCTTTT	TGACTCCATATACCATATAATTGC	45.0	33.3	58.1	54.1	1584-1607 bp	0.269231	11.9
rpl16_p4	TTTTCACAGGGCCCCTTTTC	TGACTCCATATACCATATAATTGCT	50.0	32.0	58.6	55.5	1583-1606 bp	0.269231	11.9
rpl16_p5	TTTTTCACAGGGCCCCTTTT	GACTCCATATACCATATAATTGCT	45.0	33.3	58.1	53.8	1583-1606 bp	0.269231	11.9