Hotspot

ndhF-trnL-UAG

SSC · 113625-114485 · 861 bp

Back to Delphinium

Pi 0.0182

MarkerSeek score 71.00

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 861
variable_sites: 93
pi: 0.0182
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	GGTATGATCCACGCATATTGA	AGCGTGTCTACCGATTTCAC	42.9	50.0	55.6	58.0	660-953 bp	1.0	65.0
ndhF-trnL-UAG_p2	GGTATGATCCACGCATATTGA	CAGCGTGTCTACCGATTTCA	42.9	50.0	55.6	58.0	661-954 bp	1.0	64.9
ndhF-trnL-UAG_p3	GGTATGATCCACGCATATTGA	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	55.6	57.9	670-963 bp	1.0	64.0
ndhF-trnL-UAG_p4	GGTATGATCCACGCATATTGA	TTATAAGATGCCGCCACTCG	42.9	50.0	55.6	57.9	719-1012 bp	1.0	63.6
ndhF-trnL-UAG_p5	GGTATGATCCACGCATATTGA	CTAAGAGCAGCGTGTCTACC	42.9	55.0	55.6	57.8	668-961 bp	1.0	63.5