Hotspot

rpl33-rps18

LSC · 68084-68256 · 173 bp

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Pi 0.0389

MarkerSeek score 76.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 173
variable_sites: 30
pi: 0.0389
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	AATCAATTCGATCCCCCGAC	55.0	50.0	58.5	57.7	343-1380 bp	1.0	83.4
rpl33-rps18_p2	GCATACGATTCATGGGGAGA	AATCAATTCGATCCCCCGAC	50.0	50.0	57.2	57.7	287-1378 bp	1.0	81.6
rpl33-rps18_p3	AGCATACGATTCATGGGGAG	AATCAATTCGATCCCCCGAC	50.0	50.0	57.2	57.7	288-1378 bp	1.0	81.5
rpl33-rps18_p4	GACACAATACGCCTAGTCGA	AATCAATTCGATCCCCCGAC	50.0	50.0	57.2	57.7	342-1379 bp	1.0	81.0
rpl33-rps18_p5	TCGACACAATACGCCTAGTC	AATCAATTCGATCCCCCGAC	50.0	50.0	57.2	57.7	344-1378 bp	1.0	81.0