Hotspot

trnP-UGG-psaJ

LSC · 66663-67350 · 688 bp

Back to Delphinium

Pi 0.0271
MarkerSeek score 80.90
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
688
variable_sites
107
pi
0.0271
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 80-903 bp 1.0 89.6
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 80-902 bp 1.0 89.6
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 80-914 bp 1.0 89.5
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 80-913 bp 1.0 89.5
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG AATGTCAACGCATCTGGGAA 50.0 45.0 58.1 57.8 82-916 bp 1.0 89.5