Hotspot

petA-psbJ

LSC · 63449-63891 · 443 bp

Back to Delphinium

Pi 0.021

MarkerSeek score 73.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 48
pi: 0.021
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCATCTGTTATTCTGGCA	ATTCTGGGTTGGGTTCATCC	45.0	50.0	57.2	57.5	546-558 bp	0.961538	77.8
petA-psbJ_p2	TTGGCATCTGTTATTCTGGCA	ATTCTGGGTTGGGTTCATCC	42.9	50.0	57.9	57.5	547-559 bp	0.961538	75.5
petA-psbJ_p3	GGCATCTGTTATTCTGGCAC	ATTCTGGGTTGGGTTCATCC	50.0	50.0	56.9	57.5	545-557 bp	0.961538	75.1
petA-psbJ_p4	TGGCATCTGTTATTCTGGCA	TGGCTTATAGGCACTGTAGC	45.0	50.0	57.2	57.1	613-625 bp	0.961538	73.8
petA-psbJ_p5	TGGCATCTGTTATTCTGGCA	GGCACTGTAGCTGGTATTCT	45.0	50.0	57.2	57.0	604-616 bp	0.961538	73.3