Hotspot

rbcL-accD

LSC · 57000-57705 · 706 bp

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Pi 0.00807

MarkerSeek score 68.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 42
pi: 0.00807
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCAAATGGAGCCTTGAACT	TAGCCCACACCTGTGTTCTA	45.0	50.0	58.0	58.0	832-858 bp	0.961538	86.1
rbcL-accD_p2	AATGGAGCCTTGAACTAGCC	TAGCCCACACCTGTGTTCTA	50.0	50.0	57.6	58.0	828-854 bp	0.961538	82.7
rbcL-accD_p3	TGCAAATGGAGCCTTGAACT	CTTAGCCCACACCTGTGTTC	45.0	55.0	58.0	58.5	834-860 bp	0.961538	82.4
rbcL-accD_p4	TGCAAATGGAGCCTTGAACT	GGGACCAATACCTTCTAGCG	45.0	55.0	58.0	57.5	859-885 bp	0.961538	82.4
rbcL-accD_p5	TGCAAATGGAGCCTTGAACT	TACTTAGCCCACACCTGTGT	45.0	50.0	58.0	58.3	836-862 bp	0.923077	78.9