Hotspot

ndhC-trnV-UAC

LSC · 50727-51796 · 1070 bp

Back to Delphinium

Pi 0.014

MarkerSeek score 70.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1070
variable_sites: 85
pi: 0.014
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGCCAAAACTCCGGAAATT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	59.0	57.8	1007-1236 bp	1.0	80.0
ndhC-trnV-UAC_p2	TAATTGGGGCCAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1013-1226 bp	0.923077	78.5
ndhC-trnV-UAC_p3	TAATTGGGGCCAAAACTCCG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.4	57.5	1012-1225 bp	0.923077	75.8
ndhC-trnV-UAC_p4	TAATTGGGGCCAAAACTCCG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.4	58.7	1001-1214 bp	0.923077	74.7
ndhC-trnV-UAC_p5	TAATTGGGGCCAAAACTCCG	AAGGTCTACGGTTCGAGTCC	50.0	55.0	58.4	58.8	1011-1224 bp	0.923077	73.5