Hotspot

ycf3-trnS-GGA

LSC · 44264-45130 · 867 bp

Back to Delphinium

Pi 0.0118

MarkerSeek score 71.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 63
pi: 0.0118
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	945-962 bp	1.0	79.0
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	916-933 bp	1.0	78.5
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	966-983 bp	1.0	78.4
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	946-963 bp	1.0	76.7
ycf3-trnS-GGA_p5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.6	58.1	943-960 bp	1.0	75.9