Hotspot

trnT-GGU-psbD

LSC · 31129-32681 · 1553 bp

Back to Delphinium

Pi 0.0161

MarkerSeek score 78.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1553
variable_sites: 148
pi: 0.0161
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1628-1739 bp	1.0	88.2
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	GACCGGACCAACCTACAAAA	50.0	50.0	57.9	57.7	1642-1753 bp	1.0	88.1
trnT-GGU-psbD_p3	ACAGGCCCTTTTAACTCAGC	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1632-1743 bp	0.923077	81.7
trnT-GGU-psbD_p4	ACAGGCCCTTTTAACTCAGC	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1646-1757 bp	0.923077	81.6
trnT-GGU-psbD_p5	ACAGGCCCTTTTAACTCAGC	TAGACCGGACCAACCTACAA	50.0	50.0	58.1	57.7	1648-1759 bp	0.923077	81.2