Hotspot

trnC-GCA-petN

LSC · 26229-27114 · 886 bp

Back to Delphinium

Pi 0.0119

MarkerSeek score 69.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 886
variable_sites: 74
pi: 0.0119
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	930-959 bp	1.0	88.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	924-953 bp	1.0	87.3
trnC-GCA-petN_p3	TTTATCGTTTTGGCGGCATG	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.7	58.0	983-1012 bp	1.0	86.7
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	948-977 bp	1.0	86.4
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	984-1013 bp	1.0	85.5